Depression is common among early childhood education (ECE) staff and can impair children’s learning. Among adults, greater spirituality is associated with a lower risk of depression, while adverse childhood experiences (ACEs) are associated with a higher risk. The purpose of this study was to determine whether ECE staff who reported more frequent daily spiritual experiences had a lower prevalence of depression, and whether this association was present across a range of exposures to ACEs. In the 2012 Pennsylvania Head Start Staff Wellness Survey, we collected data from 2105 Head Start and Early Head Start staff on their daily spiritual experiences (Daily Spiritual Experience Scale [DSES] score), ACE scores (count of exposure to eight categories of childhood adversity), and depression (Center for Epidemiologic Studies Depression Scale score ≥16). (author abstract)